Stripe rust and leaf rust are among the most devastating diseases of wheat, limiting its production globally. Wheat wild relatives harbour genetic diversity for new genes and...

Fusarium head blight (FHB), caused by the fungus Fusarium graminearum, is an economically devastating disease of wheat worldwide. Fhb1, a widely used genetic source of FHB...

Leaf rust and stripe rust are among the most damaging diseases of wheat worldwide. Wheat cultivation based on limited number of rust resistance genes deployed over vast areas...

Powdery mildew is a severe disease in wheat. In barley, durable resistance exists, based on non-functionality of the Mlo gene. As a model to analyse the effects of mutagenesis...

One of the most important tools to identify and validate rust resistance gene function is by producing loss-of-function mutants. Mutants can be produced using irradiation,...

Variations in wheat yield are largely explained by changes in grain number per m2 which is linked to the number of fertile florets at anthesis. This, in turn is the outcome of...

The genome sequence and analysis of wheat’s progenitor provides a roadmap to enhance genomics-assisted breeding and improvement of modern wheat varieties.

Earliness per se (Eps) genes are critical for fine-tuning flowering time and likely to modify other developmental traits as well. Effects of Eps alleles on components comprising...

Kahiluoto et al. assert that climate resilience in European wheat has declined due to current breeding practices. To support this alarming claim, the authors report yield...

MutChromSeq is an approach for isolation of genes and DNA sequences controlling gene expression in plants with complex and polyploid genomes. It involves a lossless complexity...

MutRenSeq is a method to clone disease resistance (R) genes in plants. Tips and detailed experimental protocols for the pipeline, including the complexity reduction by R gene...

Traditionally, diagnostic tools for plant pathogens were limited to the analysis of purified pathogen isolates subjected to phenotypic characterization and/or PCR-based...

DNA is widely used in plant genetic and molecular biology studies. In this chapter, we describe how to extract DNA from wheat tissues. The tissue samples are ground to disrupt...

Variation in photoperiod sensitivity in wheat plays a major role in the crop adaptation to wide agronomic environments. Photoperiod insensitivity is provided by Ppd-Aa, Ppd-B1a...

Crop diseases reduce wheat yields by ~25% globally and thus pose a major threat to global food security1. Genetic resistance can reduce crop losses in the field and can be...

Aegilops peregrina, a wild tetraploid relative of wheat with genome constitution UUSS, displays genetic variation for resistance to leaf and stripe (yellow) rust. The wheat Ae....