Generation of Loss-of-Function Mutants for Wheat Rust Disease Resistance Gene Cloning

One of the most important tools to identify and validate rust resistance gene function is by producing loss-of-function mutants. Mutants can be produced using irradiation, chemicals, and insertions. Among all the mutagens, ethyl methanesulfonate (EMS) and sodium azide are most favored because of the ease of use and generation of random point mutations in the genome. The mutants so produced facilitate the isolation, identification and cloning of rust resistance genes. In this chapter we describe a protocol for seed mutagenesis of wheat with EMS and sodium azide.

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Author Mago, Rohit
Last Updated November 21, 2019, 18:05 (UTC)
Created July 31, 2019, 16:29 (UTC)
Article Is Open Access false
Citation Report https://scite.ai/reports/10.1007/978-1-4939-7249-4_17
DFW Organisation JIC
DFW Work Package 2
DOI 10.1007/978-1-4939-7249-4_17
Journal Is Open Access false
Open Access Status closed
Publisher URL https://link.springer.com/protocol/10.1007%2F978-1-4939-7249-4_17