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Genetic Transformation of Common Wheat (Triticum aestivum L.) Using Biolistics
The following protocol describes the genetic transformation of wheat using the BioRad PDS/1000-He particle delivery system. Immature embryos are isolated 12–16 days... -
The Importance of Wheat
The history of wheat domestication and use is closely linked to the efforts of humans to protect themselves from hunger and gain control over their food supply. Now grown... -
FODMAPs in Wheat
The dietary intake of fermentable oligo-, di- and monosaccharides, and polyols (FODMAPs) can promote gut health, but also trigger gastrointestinal disorders. Wheat as a staple... -
Health Hazards Associated with Wheat and Gluten Consumption in Susceptible In...
Wheat accounts for about 20% to over 50% of the total calorie intake of food in regions where it is grown. However, there is a clear perception that disorders related to the... -
Generation of Loss-of-Function Mutants for Wheat Rust Disease Resistance Gene...
One of the most important tools to identify and validate rust resistance gene function is by producing loss-of-function mutants. Mutants can be produced using irradiation,... -
High-Density SNP Genotyping Array for Hexaploid Wheat and Its Relatives
A lack of genetic diversity between wheat breeding lines has been recognized as a significant block to future yield increases. Wheat breeding and prebreeding strategies are... -
Rapid Gene Isolation Using MutChromSeq
MutChromSeq is an approach for isolation of genes and DNA sequences controlling gene expression in plants with complex and polyploid genomes. It involves a lossless complexity... -
MutRenSeq: A Method for Rapid Cloning of Plant Disease Resistance Genes
MutRenSeq is a method to clone disease resistance (R) genes in plants. Tips and detailed experimental protocols for the pipeline, including the complexity reduction by R gene... -
Field Pathogenomics: An Advanced Tool for Wheat Rust Surveillance
Traditionally, diagnostic tools for plant pathogens were limited to the analysis of purified pathogen isolates subjected to phenotypic characterization and/or PCR-based... -
Isolation of Wheat Genomic DNA for Gene Mapping and Cloning
DNA is widely used in plant genetic and molecular biology studies. In this chapter, we describe how to extract DNA from wheat tissues. The tissue samples are ground to disrupt...
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