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LYS3 encodes a prolamin-box-binding transcription factor that controls embryo...
Mutations at the LYS3 locus in barley have multiple effects on grain development, including an increase in embryo size and a decrease in endosperm starch content. The gene... -
Optimisation of droplet digital PCR for determining copy number variation of ...
Targeted and random mutagenesis of gene families require accurate quantification. Droplet digital PCR (ddPCR) enables high-throughput screening of copy number variation (CNV).... -
Development of the GlutEnSeq capture system for sequencing gluten gene famili...
We developed an in-solution gluten exome capture system called GlutEnSeq (Gluten gene Enrichment and Sequencing), covering the sequence variation of thousands of prolamin genes... -
Food processing and breeding strategies for coeliac-safe and healthy wheat pr...
A strict gluten-free diet is currently the only treatment for the 1-2% of the world population who suffer from coeliac disease (CD). However, due to the presence of wheat and... -
Barley lys3 mutants are unique amongst shrunken-endosperm mutants in having a...
Many shrunken endosperm mutants of barley (Hordeum vulgare L.) have been described and several of these are known to have lesions in starch biosynthesis genes. Here we confirm...
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