The CerealsDB website, created by members of the Functional Genomics Group at the University of Bristol, provides access to a database containing SNP and genotyping data for...

Efficient use of nitrogen (N) by plants and particularly crops, is of global importance. In agriculture, high crop yields and protein content depend upon extensive N-inputs,...

The following protocol describes the genetic transformation of wheat using the BioRad PDS/1000-He particle delivery system. Immature embryos are isolated 12–16 days...

The history of wheat domestication and use is closely linked to the efforts of humans to protect themselves from hunger and gain control over their food supply. Now grown...

The dietary intake of fermentable oligo-, di- and monosaccharides, and polyols (FODMAPs) can promote gut health, but also trigger gastrointestinal disorders. Wheat as a staple...

Wheat accounts for about 20% to over 50% of the total calorie intake of food in regions where it is grown. However, there is a clear perception that disorders related to the...

One of the most important tools to identify and validate rust resistance gene function is by producing loss-of-function mutants. Mutants can be produced using irradiation,...

Wheat is a major world food crop and its production needs to increase to meet future demand. However, having a large complex genome, the analysis of bread wheat is not without...

Acrylamide is a processing contaminant that forms from free asparagine and reducing sugars, such as glucose, fructose and maltose, during high-temperature cooking and...

A lack of genetic diversity between wheat breeding lines has been recognized as a significant block to future yield increases. Wheat breeding and prebreeding strategies are...

Bread wheat (Triticum aestivum) is grown across a wide range of environments having been selected in part for a complex of genes controlling adaptive response. As a major...

MutChromSeq is an approach for isolation of genes and DNA sequences controlling gene expression in plants with complex and polyploid genomes. It involves a lossless complexity...

MutRenSeq is a method to clone disease resistance (R) genes in plants. Tips and detailed experimental protocols for the pipeline, including the complexity reduction by R gene...

Traditionally, diagnostic tools for plant pathogens were limited to the analysis of purified pathogen isolates subjected to phenotypic characterization and/or PCR-based...

DNA is widely used in plant genetic and molecular biology studies. In this chapter, we describe how to extract DNA from wheat tissues. The tissue samples are ground to disrupt...

Acrylamide (C3H5NO) is a processing contaminant formed from free asparagine and reducing sugars during high-temperature cooking and processing. It is a Group 2A carcinogen, and...