Rapid Gene Isolation Using MutChromSeq

MutChromSeq is an approach for isolation of genes and DNA sequences controlling gene expression in plants with complex and polyploid genomes. It involves a lossless complexity reduction by flow cytometric chromosome sorting and shotgun sequencing DNA from isolated chromosomes. Comparison of sequences from wild-type parental chromosome with chromosomes from multiple independently derived mutants identifies causative mutations in a single candidate gene or a noncoding sequence. MutChromSeq does not rely on recombination-based genetic mapping and does not exclude any DNA sequence from being targeted.

Data a zdroje

Tato datová sada neobsahuje data

Doplňující informace

Pole Hodnota
Zdroj
Verze
Authors
Správce
Email správce
Article Is Open Access false
Citation Report https://scite.ai/reports/10.1007/978-1-4939-7249-4_20
DFW Organisation JIC
DFW Work Package 2
DOI 10.1007/978-1-4939-7249-4_20
Journal Is Open Access false
Open Access Status closed