In vitro fermentability of xylo -oligosaccharide and xylo -polysaccharide fractions with different molecular weights by human faecal bacteria

Xylo-oligosaccharides and xylo-polysaccharides (XOS, XPS) produced by autohydrolysis of the fibre from oil palm empty fruit bunches (OPEFB) were purified using gel filtration chromatography to separate the XOS and XPS from the crude autohydrolysis liquor. Six mixed fractions of refined XOS and XPS with average degree of polymerisation (avDP) of 4–64 were obtained. These were characterised in terms of their composition and size by HPLC, MALDI-ToF-MS (selected fractions) and carbohydrate gel electrophoresis (PACE). They were assessed in batch culture fermentations using faecal inocula to determine their ability to modulate the human faecal microbiota in vitro by measuring the bacterial growth, organic acid production and the XOS assimilation profile. The gut microbiota was able to utilise all the substrates and there was a link between the avDP with the fermentation properties. In general, XOS/XPS preparations of lower avDP promote better Bifidobacterium growth and organic acid production.

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Citation Report https://scite.ai/reports/10.1016/j.carbpol.2017.08.077
DFW Organisation RRes
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DOI 10.1016/j.carbpol.2017.08.077
Date Last Updated 2019-07-27T22:17:05.534991
Evidence oa repository (via OAI-PMH title and first author match)
Journal Is Open Access false
Open Access Status green
PDF URL http://centaur.reading.ac.uk/73126/1/Ho%20et%20al%20Centaur.pdf
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