Epigenetic switch reveals CRISPR/Cas9 response to cytosine methylation in plants

Genome editing techniques, such as the CRISPR/Cas9 system, offer a game-changing opportunity for crop improvement by enabling precise modifications to be made at targeted genomic loci. The CRISPR/Cas9 system has been employed successfully in many plant species; however, in order to use the system to its full potential, it is important to understand precisely how it functions and the factors that may limit its effectiveness. The mechanistic details of Cas9-induced double-strand breaks (DSBs), that underpin the mutational ability of the system, have been well-described. It also is known that the efficiency of editing varies for different target sequences. However, the impact of epigenetic modifications on CRISPR/Cas9 efficacy and subsequent DNA repair is poorly understood, especially in plants.

Data and Resources

Additional Info

Field Value
  • Name: Raffan, Sarah, Type: Corresponding Author,
  • Name: Kaur, Navneet, Type: Author,
  • Name: Halford, Nigel G., Type: Author,
Maintainer Email
Article Host Type repository
Article Is Open Access true
Article License Type
Article Version Type submittedVersion
Citation Report https://scite.ai/reports/10.1111/nph.18405
DFW Organisation RRes
DFW Work Package 1
DOI 10.1111/nph.18405
Date Last Updated 2022-08-20T02:40:38.161952
Evidence oa repository (via OAI-PMH doi match)
Funder Code(s)
Journal Is Open Access false
Open Access Status green
PDF URL https://repository.rothamsted.ac.uk/download/81d6920b8c1040aef0324f49587f062c14a71beefeeb18b6b10c3247ea2c2bdb/5803180/Raffan%2C%20Kaur%2C%20Halford%2C%20New%20Phytol%202022.pdf
Publisher URL https://nph.onlinelibrary.wiley.com/doi/10.1111/nph.18405