Rapid Gene Isolation Using MutChromSeq

MutChromSeq is an approach for isolation of genes and DNA sequences controlling gene expression in plants with complex and polyploid genomes. It involves a lossless complexity reduction by flow cytometric chromosome sorting and shotgun sequencing DNA from isolated chromosomes. Comparison of sequences from wild-type parental chromosome with chromosomes from multiple independently derived mutants identifies causative mutations in a single candidate gene or a noncoding sequence. MutChromSeq does not rely on recombination-based genetic mapping and does not exclude any DNA sequence from being targeted.

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Additional Info

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Author Steuernagel, Burkhard
Last Updated August 7, 2019, 14:00 (UTC)
Created July 31, 2019, 16:29 (UTC)
Article Is Open Access false
Citation Report https://scite.ai/reports/10.1007/978-1-4939-7249-4_20
DFW Organisation JIC
DFW Work Package 2
DOI 10.1007/978-1-4939-7249-4_20
Journal Is Open Access false
Open Access Status closed