Efficient CRISPR/Cas-mediated Targeted Mutagenesis in Spring and Winter Wheat Varieties

AbstractThe CRISPR/Cas technology has recently become a molecular tool of choice for gene function studies in plants as well as crop improvement. Wheat is a globally important staple crop with a well annotated genome and there is plenty of scope for improving its agriculturally important traits using genome editing technologies, such as CRISPR/Cas. As part of this study we targeted three different genes in hexaploid wheat Triticum aestivum: TaBAK1-2 in the spring cultivar Cadenza as well as Ta-eIF4E and Ta-eIF(iso)4E in winter cultivars Cezanne, Goncourt and Prevert. The primary transgenic lines carrying CRISPR/Cas-induced indels were successfully generated for all targeted genes. As winter wheat varieties are generally less amenable to genetic transformation, the successful experimental methodology for transformation and genome editing in winter wheat presented in this study will be of interest to the research community working with this crop.

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  • Name: Hahn, Florian, Type: Corresponding Author,
  • Name: Loures, Laura Sanjurjo, Type: Author,
  • Name: Sparks, Caroline A., Type: Author,
  • Name: Kanyuka, Kostya, Type: Author,
  • Name: Nekrasov, Vladimir, Type: Author,
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Citation Report https://scite.ai/reports/10.3390/plants10071481
DFW Organisation RRes
DFW Work Package 1
DOI 10.3390/plants10071481
Date Last Updated 2022-09-16T14:38:15.330251
Evidence oa journal (via doaj)
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Journal Is Open Access true
Open Access Status gold
PDF URL https://www.mdpi.com/2223-7747/10/7/1481/pdf
Publisher URL https://doi.org/10.3390/plants10071481