Rapid Generation and Analysis of a Barley Doubled Haploid Line with Higher Nitrogen Use Efficiency Than Parental Lines by F1 Microspore Embryogenesis

Creating varieties with high nitrogen use efficiency (NUE) is crucial for sustainable agriculture development. In this study, a superior barley doubled haploid line (named DH45) with improved NUE was produced via F1 microspore embryogenesis with three rounds of screening in different nitrogen levels by hydroponic and field experiments. The molecular mechanisms responsible for the NUE of DH45 surpassing that of its parents were investigated by RNA-seq analysis. A total of 1027 differentially expressed genes (DEGs) were identified that were up- or down-regulated in DH45 under low nitrogen conditions but showed no significant differences in the parents. GO analysis indicated that genes involved in nitrogen compound metabolic processes were significantly enriched in DH45 compared with the parents. KEGG analysis showed the MAPK signaling pathway plant to be highly enriched in DH45 relative to its parents, as well as genes involved in alanine, aspartate and glutamate metabolism, and arginine biosynthesis. In conclusion, our study revealed the potential to fix trait superiority in a line by combining crossing with F1 microspore culture technologies in future crop breeding and also identified several candidate genes that are expressed in shoots and may enable barley to cope with low-nitrogen stress.

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  • Name: Xu, Hongwei, Type: Corresponding Author,
  • Name: Li, Yingbo, Type: Author,
  • Name: Gao, Runhong, Type: Author,
  • Name: Xu, Rugen, Type: Author,
  • Name: Guo, Guimei, Type: Author,
  • Name: Lu, Ruiju, Type: Author,
  • Name: Halford, Nigel G., Type: Author,
  • Name: Chen, Zhiwei, Type: Author,
  • Name: Liu, Chenghong, Type: Author,
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Article Host Type publisher
Article Is Open Access true
Article License Type cc-by
Article Version Type publishedVersion
Citation Report https://scite.ai/reports/10.3390/plants10081588
DFW Organisation RRes
DFW Work Package 1
DOI 10.3390/plants10081588
Date Last Updated 2021-09-10T12:22:32.047027
Evidence oa journal (via doaj)
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Journal Is Open Access true
Open Access Status gold
PDF URL https://www.mdpi.com/2223-7747/10/8/1588/pdf
Publisher URL https://doi.org/10.3390/plants10081588