Characterization of an antimicrobial and phytotoxic ribonuclease secreted by the fungal wheat pathogen Zymoseptoria tritici

The fungus Zymoseptoria tritici is the causal agent of Septoria Tritici Blotch (STB) disease of wheat leaves. Zymoseptoria tritici secretes many functionally uncharacterized effector proteins during infection. Here, we characterized a secreted ribonuclease (Zt6) with an unusual biphasic expression pattern. Transient expression systems were used to characterize Zt6, and mutants thereof, in both host and non‐host plants. Cell‐free protein expression systems monitored the impact of Zt6 protein on functional ribosomes, and in vitro assays of cells treated with recombinant Zt6 determined toxicity against bacteria, yeasts and filamentous fungi. We demonstrated that Zt6 is a functional ribonuclease and that phytotoxicity is dependent on both the presence of a 22‐amino‐acid N‐terminal ‘loop’ region and its catalytic activity. Zt6 selectively cleaves both plant and animal rRNA species, and is toxic to wheat, tobacco, bacterial and yeast cells, but not to Z. tritici itself. Zt6 is the first Z. tritici effector demonstrated to have a likely dual functionality. The expression pattern of Zt6 and potent toxicity towards microorganisms suggest that, although it may contribute to the execution of wheat cell death, it is also likely to have an important secondary function in antimicrobial competition and niche protection.

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Author Kettles, Graeme J.
Last Updated August 7, 2019, 10:55 (UTC)
Created July 31, 2019, 16:30 (UTC)
Article Host Type publisher
Article Is Open Access true
Article License Type cc-by
Article Version Type publishedVersion
Citation Report https://scite.ai/reports/10.1111/nph.14786
DFW Organisation RRes
DFW Work Package 2
DOI 10.1111/nph.14786
Date Last Updated 2018-12-31T21:56:21.983232
Evidence open (via page says license)
Journal Is Open Access false
Open Access Status hybrid
PDF URL https://nph.onlinelibrary.wiley.com/doi/pdf/10.1111/nph.14786
Publisher URL https://doi.org/10.1111/nph.14786