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Aegilops umbellulata introgression carrying leaf rust and stripe rust resista...
Leaf rust and stripe rust are among the most damaging diseases of wheat worldwide. Wheat cultivation based on limited number of rust resistance genes deployed over vast areas... -
Development of mlo-based resistance in tetraploid wheat against wheat powdery...
Powdery mildew is a severe disease in wheat. In barley, durable resistance exists, based on non-functionality of the Mlo gene. As a model to analyse the effects of mutagenesis... -
Generation of Loss-of-Function Mutants for Wheat Rust Disease Resistance Gene...
One of the most important tools to identify and validate rust resistance gene function is by producing loss-of-function mutants. Mutants can be produced using irradiation,... -
Rapid Gene Isolation Using MutChromSeq
MutChromSeq is an approach for isolation of genes and DNA sequences controlling gene expression in plants with complex and polyploid genomes. It involves a lossless complexity... -
MutRenSeq: A Method for Rapid Cloning of Plant Disease Resistance Genes
MutRenSeq is a method to clone disease resistance (R) genes in plants. Tips and detailed experimental protocols for the pipeline, including the complexity reduction by R gene... -
Field Pathogenomics: An Advanced Tool for Wheat Rust Surveillance
Traditionally, diagnostic tools for plant pathogens were limited to the analysis of purified pathogen isolates subjected to phenotypic characterization and/or PCR-based... -
Isolation of Wheat Genomic DNA for Gene Mapping and Cloning
DNA is widely used in plant genetic and molecular biology studies. In this chapter, we describe how to extract DNA from wheat tissues. The tissue samples are ground to disrupt... -
BED-domain-containing immune receptors confer diverse resistance spectra to y...
Crop diseases reduce wheat yields by ~25% globally and thus pose a major threat to global food security1. Genetic resistance can reduce crop losses in the field and can be... -
Fine mapping of Aegilops peregrina co-segregating leaf and stripe rust resist...
Aegilops peregrina, a wild tetraploid relative of wheat with genome constitution UUSS, displays genetic variation for resistance to leaf and stripe (yellow) rust. The wheat Ae....
